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KMID : 1025520040460060909
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Journal of Animal Science and Technology
2004 Volume.46 No. 6 p.909 ~ p.916
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Molecular Cloning and mRNA Expression of the Hanwoo CCAAT/enhancer-binding Protein α(C/EBPα) Gene
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Jeoung Y. H.
Lee Sang-Mi
Pack Hyo-Young
Yoon Du-Hak
Moon Seung-Ju
Chung Eui-Ryong
Kang Man-Jong
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Abstract
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CCAAT/enhancer binding proteins(C/EBP) are a group of transcription factors expressed during preadipocyte differentiation. In the C/EBPs, C/EBPα plays an important role in lipid deposition and adipocyte differentiation. In this studies, we report the identification, characterization, and expression of a Hanwoo C/EBPα. The Hanwoo C/EBPα DNA includes a 1059 bp open reading frame encoding a protein of 353 amino acids. The C/EBPα amino acid sequences of the Hanwoo show strong conservation with the corresponding sequences reported in other species. The distribution of C/EBPα mRNA in various tissues of Hanwoo aged 12 months were investigated using Northern blotting analysis. The highest expression was detected in adipose tissue and more lower expression was detected in colon and lung. We also identified expression of C/EBPα mRNA in Hanwoo sirloin and adipose tissue aged 12, 26, and 30 months by real-time RT-PCR. The higest expression were detected at 26 months in the sirloin and at 12 and 26 months in the adipose tissue.
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KEYWORD
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Adipocyte differentiation, C/EBPα, Hanwoo
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